Tg(CD2-Tbx21)709Staka
Transgene Detail

Summary

• Symbol: Tg(CD2-Tbx21)709Staka
• Name: transgene insertion 709, Satoru Takahashi
• MGI ID: MGI:3777809
• Synonyms: T-bet-tg, VA T-bet line709
• Transgene: Tg(CD2-Tbx21)709Staka Location: unknown
• Alliance: Tg(CD2-Tbx21)709Staka page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD2-Tbx21)709Staka expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tbx21 (MGI:1888984)

• Mutation details: A full-length, 2.5-kb mouse Tbx21 cDNA was inserted into the VA CD2 expression vector between, 5', the promoter, ATG-free mutant first exon and first intron of the human CD2 gene and 3', also from the CD2 gene, ~500 bp of 3' untranslated and flanking DNA including two polyadenylation signals and the 3' locus control region. A lacZ reporter gene was shown to be expressed from this vector in mature peripheral CD4⁺CD8^- and CD4^-CD8⁺ SP T lymphocytes and in peripheral CD4^-CD8^- DN T cells. (J:105228, J:128630)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This particular transgenic line is not specifically described in the literature. Ishizaki et al. (J:133041) report generation of Tg lines 725 and 731 and crossing them to C57BL/6 for 4 generations. Kiwamoto et al. (J:128630) do not identify the transgenic lines used and says they were backcrossed to BALB/c for 8 generations.

References

• Original: J:128630 Kiwamoto T, et al., Transcription factors T-bet and GATA-3 regulate development of airway remodeling. Am J Respir Crit Care Med. 2006 Jul 15;174(2):142-51
• All: 4 reference(s)