Gt(ROSA)26Sor^{tm4(CAG-lacZ,-EGFP)Dym}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(CAG-lacZ,-EGFP)Dym}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Susan M Dymecki
• MGI ID: MGI:3777625
• Synonyms: Gt(ROSA)26Sor^tm4Dym, RC::PFwe
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(CAG-lacZ,-EGFP)Dym} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133039
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A dual recombinase-responsive indicator cassette was knocked into the gene locus. This cassette consisted of (upstream to downstream): a cytomegalovirus enhancer/chicken beta-actin promoter; a splice acceptor; a loxP-flanked PGK-neomycin resistance cassette followed by four consecutive polyadenylation sequences; a FRT-flanked nuclear lacZ encoding gene followed by five consecutive polyadenylation sequences; and a bicistronic element encoding for wheat germ agglutinin (WGA) and a farnesylated, membrane-localized form of eGFP. This allele expresses nlacZ after cre-mediated excision of the neo cassette and turns off nlacZ and turns on WGA and eGFP expression after both cre- and Flp-recombinases are expressed. (J:133039)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:133039 Farago AF, et al., Assembly of the brainstem cochlear nuclear complex is revealed by intersectional and subtractive genetic fate maps. Neuron. 2006 Apr 20;50(2):205-18
• All: 7 reference(s)