Tcrb^tm2(Tcrb)Fwa
Targeted Allele Detail

Summary

• Symbol: Tcrb^tm2(Tcrb)Fwa
• Name: T cell receptor beta chain; targeted mutation 2, Frederick W Alt
• MGI ID: MGI:3776870
• Synonyms: Jbeta1^M7, Vbeta14/3DbetaRS
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132446
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: ES cells containing the Tcrb^tm3Slekmutation that lack the Dbeta2-Jbeta2 cluster on both alleles and have a deletion of the Dbeta1 gene segment on one allele, were used for further targeting. The upstream recombination signaling sequence (RSS) for the Vbeta14 gene segment was replaced with the upstream RSS from the Dbeta1 gene. A floxed neomycin resistance cassette used for targeting was removed by transient expression of Cre recombinase leaving a single loxP site 172 bp upstream of the knocked in RSS. Only clones that had the targeted mutation in trans to the Dbeta1 gene segment were selected. Because the Dbeta1 mutation prevents recombination of the Vbeta14 gene segment, mice derived from these stem cells are functionally haploid for the Vbeta14 gene segment with the knocked in RSS. ES cells with the desired targeting event were confirmed by Southern blotting, and chimeric mice were generated by Rag2-deficient blastocyte complementation. (J:132446)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 94 strains or lines available

References

• Original: J:132446 Wu C, et al., Dramatically increased rearrangement and peripheral representation of Vbeta14 driven by the 3'Dbeta1 recombination signal sequence. Immunity. 2003 Jan;18(1):75-85
• All: 2 reference(s)