Gt(ROSA)26Sor^{tm1(CAG-cre)Jphe}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-cre)Jphe}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Jean-Paul Herman
• MGI ID: MGI:3774388
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-cre)Jphe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130959
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: rapamycin
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-cre)Jphe} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The expression of a dimerizable cre was achieved by insertion of the cre60.F2 N-terminal cre moiety which is fused to FRB (the ligand binding domain of FRAP,an FKBP12-rapamycin associated protein) under the control of the endogenous promoter and a Caggs-cre59.F2 cassette, containing the C-teminal moiety of cre fused to FKBP12 (FK506-binding protein) under control of the CAG promoter, into the ROSA locus. A hygro cassette was also inserted into the locus for selection purposes. The split cre is inactive until dimerization is induced by administration of rapamycin. (J:130959)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:130959 Jullien N, et al., Conditional Transgenesis Using Dimerizable Cre (DiCre). PLoS One. 2007;2(12):e1355
• All: 1 reference(s)