Igh^{tm1(VH558)Mss}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm1(VH558)Mss}
• Name: immunoglobulin heavy chain complex; targeted mutation 1, Mark S Schlissel
• MGI ID: MGI:3773307
• Synonyms: VH-KI
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130421
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A variable heavy chain gene segment belonging to the VH558 family along with 1.3 kb of upstream sequence and 500 bp of downstream sequence was knocked into a region of the IgH complex that lies 500 bp upstream of the most proximal D segment, DFL16.1. A loxP-flanked neomycin targeting cassette included in the targeting was subsequently removed by crossing chimeric mice with Cre expressing transgenic mice. Targeting events were confirmed through Southern blotting and restriction mapping. (J:130421)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:130421 Bates JG, et al., Chromosomal position of a VH gene segment determines its activation and inactivation as a substrate for V(D)J recombination. J Exp Med. 2007 Dec 24;204(13):3247-56
• All: 2 reference(s)