Tg(tetO-Mcpt1)1Mrab
Transgene Detail

Summary

• Symbol: Tg(tetO-Mcpt1)1Mrab
• Name: transgene insertion 1, Marlene Rabinovitch
• MGI ID: MGI:3772577
• Synonyms: RVCH+, TRE-RVCH-HA
• Transgene: Tg(tetO-Mcpt1)1Mrab Location: unknown
• Alliance: Tg(tetO-Mcpt1)1Mrab page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Mcpt1)1Mrab expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  rat	Vch (29265)

• Mutation details: The transgene was designed with the tetracycline-responsive element (TRE; also called tet-operator or tetO) upstream of a rat vascular chymase (RVCH or Mcpt1) gene modified such that a human influenza hemagglutinin (HA) tag is added to the C terminus of mature RVCH protein. With the addition of dox expression of the RVCH-HA fusion protein can be completely abolished, reversing the hypertension phenotype. (J:70031)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:70031 Ju H, et al., Conditional and targeted overexpression of vascular chymase causes hypertension in transgenic mice. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7469-74
• All: 1 reference(s)