Ptf1a^tm2Macd
Targeted Allele Detail

Summary

• Symbol: Ptf1a^tm2Macd
• Name: pancreas specific transcription factor, 1a; targeted mutation 2, Raymond MacDonald
• MGI ID: MGI:3771319
• Synonyms: Ptf1a^{tm1.2, Ptf1aWA}
• Gene: Ptf1a Location: Chr2:19450474-19452312 bp, + strand Genetic Position: Chr2, 13.37 cM, cytoband A2
• Alliance: Ptf1a^tm2Macd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125691
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The single-codon W298A mutation was inserted into the endogenous Ptf1a locus of a mouse embryonic stem (ES) cell line by RMCE using Ptf1a^tm1Mgn. The exchange cassette contained the Ptf1a gene SalI-SalI fragment from strain 129 genomic DNA with codon 298 tryptophan (TGG) changed to alanine (GCG) by PCR mutagenesis. (J:125691)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptf1a Mutation: 30 strains or lines available

References

• Original: J:125691 Masui T, et al., Early pancreatic development requires the vertebrate Suppressor of Hairless (RBPJ) in the PTF1 bHLH complex. Genes Dev. 2007 Oct 15;21(20):2629-43
• All: 4 reference(s)