Chrnb1^tm1Sjb
Targeted Allele Detail

Summary

• Symbol: Chrnb1^tm1Sjb
• Name: cholinergic receptor nicotinic beta 1 subunit; targeted mutation 1, Steven J Burden
• MGI ID: MGI:3768925
• Synonyms: AChR-beta^3F
• Gene: Chrnb1 Location: Chr11:69674862-69686742 bp, - strand Genetic Position: Chr11, 42.87 cM
• Alliance: Chrnb1^tm1Sjb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128926
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Point mutations were introduced into the endogenous gene by homologous recombination. Codon 357 was converted from TAC to TTC, codon 390 from TAT to TTT and codon 442 from TAC to TTC. Each of these changes is predicted to alter a conserved tyrosine residue in the encoded protein to a phenylalanine residue. These amino acids are in the large intracellular loop of the protein. An frt-flanked neomycin selection cassette in intron 10 was removed via Flp-mediated recombination, leaving a single frt site in intron 10 in the final allele. Affinity purification and western blotting experiments demonstrated that the encoded protein is expressed at similar levels to controls, but does not cross-react to a phosphotyrosine-specific monoclonal antibody directed at residue 390. Similar results were seen in immunohistology experiments. (J:128926)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chrnb1 Mutation: 40 strains or lines available

References

• Original: J:128926 Friese MB, et al., Synaptic differentiation is defective in mice lacking acetylcholine receptor {beta}-subunit tyrosine phosphorylation. Development. 2007 Dec;134(23):4167-76
• All: 1 reference(s)