Trhde^tm1Lars
Targeted Allele Detail

Summary

• Symbol: Trhde^tm1Lars
• Name: TRH-degrading enzyme; targeted mutation 1, Lars Geffers
• MGI ID: MGI:3767397
• Synonyms: Trhd^-, Trhde^-, TRH-DE-
• Gene: Trhde Location: Chr10:114234725-114638207 bp, - strand Genetic Position: Chr10, 61.59 cM
• Alliance: Trhde^tm1Lars page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128543
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Insertion of a bicistronic reporter construct into exon 1 inactivated the gene and brought both lacZ and PLAP under control of the endogenous promoter. The construct contains a bacterial beta-galactosidase open reading frame followed by a retroviral internal ribosome entry site (IRES) element, a human PLAP open reading frame, and a floxed neomycin resistance gene (lacZ-IRES-PLAP-loxP-neo-loxP). In heterozygous mice, lacZ is expressed in the cerebral cortex and in the hippocampal formation of Ammon's horn. (J:128544)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trhde Mutation: 55 strains or lines available

Notes

No overt phenotype was noted in homozygous mutant mice, although these animals exhibited higher night time wheel running activity under normal light/dark cycles, and reduced activity in constant light. Reporter expression in mutant mice suggests that protein in wild-type mice would be highly expressed in photosensitive tissues.

References

• Original: J:128543 Geffers L, et al., Generation of thyrotropin-releasing hormone-degrading ectoenzyme (TRH-DE) deficient mice by targeted recombination. FENS Forum Abstracts. 2004;2:A046.5 (abstr)
• All: 3 reference(s)