Brdt^tm1Djw
Targeted Allele Detail

Summary

• Symbol: Brdt^tm1Djw
• Name: bromodomain, testis-specific; targeted mutation 1, Debra J Wolgemuth
• MGI ID: MGI:3765973
• Synonyms: Brdt^deltaBD1
• Gene: Brdt Location: Chr5:107479025-107534924 bp, + strand Genetic Position: Chr5, 52.23 cM, cytoband E5
• Alliance: Brdt^tm1Djw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128000
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 4 were replaced with a neo cassette leading to the formation of an alternative transcript from an in-frame ATG in exon 5. This shorter transcript lacks the first bromodomain. The absence of full-length protein product and the presence of a truncated protein product were confirmed by western blot analysis. (J:128000, J:190041)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brdt Mutation: 39 strains or lines available

References

• Original: J:128000 Shang E, et al., The first bromodomain of Brdt, a testis-specific member of the BET sub-family of double-bromodomain-containing proteins, is essential for male germ cell differentiation. Development. 2007 Oct;134(19):3507-15
• All: 4 reference(s)