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Plxnb1tm1Matl
Targeted Allele Detail
Summary
Symbol: Plxnb1tm1Matl
Name: plexin B1; targeted mutation 1, Marc Tessier-Lavigne
MGI ID: MGI:3765917
Synonyms: Plxnb1-, Plxnb1-lacZ
Gene: Plxnb1  Location: Chr9:108924457-108948985 bp, + strand  Genetic Position: Chr9, 59.63 cM
Alliance: Plxnb1tm1Matl page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:139221
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsTargeted mutagenesis using a promoterless gene trap vector ("targeted trapping") was used to insert a 9.4-kb placental alkaline phosphatase (PLAP) secretory cassette into intron 21. This results in transcription of a fusion mRNA joining upstream exons of the endogenous gene to the elements of the cassette, comprising a splice acceptor and a transmembrane domain coding sequence followed by the betageo (lacZ/neomycin resistance fusion) cDNA, an internal ribosomal entry site (IRES), and the human PLAP sequence with a polyadenylation signal. Two proteins are translated from this bicistronic transcript: a PLXNB1/Betageo fusion protein that is sequestered in an intracellular compartment of the neuronal cell body; and PLAP, which is expressed on the axonal surface. (Roland Freidel, data submission). Western blot of E11.5 whole embryo lysates confirmed the absence of protein in homozygous mutants. (J:228347)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 3 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Plxnb1 Mutation:  107 strains or lines available
References
Original:  J:121225 Friedel RH, et al., Gene targeting using a promoterless gene trap vector ('targeted trapping') is an efficient method to mutate a large fraction of genes. Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13188-93
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory