Tert^tm1Sear
Targeted Allele Detail

Summary

• Symbol: Tert^tm1Sear
• Name: telomerase reverse transcriptase; targeted mutation 1, Steven E Artandi
• MGI ID: MGI:3764898
• Synonyms: TERT^-
• Gene: Tert Location: Chr13:73775030-73797962 bp, + strand Genetic Position: Chr13, 40.12 cM
• Alliance: Tert^tm1Sear page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127153
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Not Specified
• Mutation details: The gene locus contains 15 exons. A linearized targeting vector containing a neomycin cassette in the reverse orientation and exons 3-8 was used. After targeting, the allele contained a neomycin cassette in the reverse orientation after the endogenous exon 8, with exons 3-8 repeated downstream of the neo cassette. Insertion caused gene inactivation as determined by semi-quantitative RT-PCR on embryonic fibroblasts, liver, and testes. (J:127153, J:150343)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tert Mutation: 55 strains or lines available

References

• Original: J:127153 Rajaraman S, et al., Telomere uncapping in progenitor cells with critical telomere shortening is coupled to S-phase progression in vivo. Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17747-52
• All: 2 reference(s)