Ptger1^tm1Matb
Targeted Allele Detail

Summary

• Symbol: Ptger1^tm1Matb
• Name: prostaglandin E receptor 1 (subtype EP1); targeted mutation 1, Matthew D Breyer
• MGI ID: MGI:3764892
• Synonyms: EP1^-
• Gene: Ptger1 Location: Chr8:84393307-84399382 bp, + strand Genetic Position: Chr8, 40.22 cM
• Alliance: Ptger1^tm1Matb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127410
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Three point mutations were introduced into exon 2 using a "hit and run" type vector. One mutation (C795T; numbering based on MGI ID: D16388) introduces an in-frame stop codon (R242X) that is predicted to stop translation at the beginning of the third cytoplasmic loop of the receptor, resulting in a nonfunctional protein. The other two mutations (C798G; G799A) introduce an EcoRI site that is used for genotyping. Sequencing of mRNA confirmed expression of the mutant allele in homozygous mice. Expression of the PKN1 gene present on the opposite antisense strand was unaffected as determined by immunoprecipitation of brain and kidney lysates. (J:127410)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptger1 Mutation: 22 strains or lines available

References

• Original: J:127410 Guan Y, et al., Antihypertensive effects of selective prostaglandin E2 receptor subtype 1 targeting. J Clin Invest. 2007 Sep;117(9):2496-505
• All: 8 reference(s)