Prnp^{tm5(PRNP*)TKit}
Targeted Allele Detail

Summary

• Symbol: Prnp^{tm5(PRNP*)TKit}
• Name: prion protein; targeted mutation 5, Tetsuyuki Kitamoto
• MGI ID: MGI:3762867
• Synonyms: Ki-Hu129M4R
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^{tm5(PRNP*)TKit} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126777
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Prnp^{tm5(PRNP*)TKit} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PRNP (5621)

  Prnp	(MGI:97769)

• Mutation details: The open reading frame was replaced with the human PRNP variant with a methionine at amino acid position 129 with four octapeptide repeats such that the mouse signal sequence was retained. Expression was confirmed by western blot analysis. (J:126777)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 143 strains or lines available

References

• Original: J:126777 Kobayashi A, et al., Cross-sequence transmission of sporadic Creutzfeldt-Jakob disease creates a new prion strain. J Biol Chem. 2007 Oct 12;282(41):30022-8
• All: 1 reference(s)