Grin1^tm1Nak
Targeted Allele Detail

Summary

• Symbol: Grin1^tm1Nak
• Name: glutamate receptor, ionotropic, NMDA1 (zeta 1); targeted mutation 1, Shigetada Nakanishi
• MGI ID: MGI:3760845
• Synonyms: NR1^-
• Gene: Grin1 Location: Chr2:25181193-25209199 bp, - strand Genetic Position: Chr2, 17.14 cM
• Alliance: Grin1^tm1Nak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:38582
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a 0.5kb genomic fragment containing coding sequence downstream of the translation initiation site codon. Northern blot analysis on RNA derived from whole brain showed that no detectable transcript is present in homozygous mice. Western blot analysis showed that no encoded protein was present in brain extracts from homozygous mice. (J:38582)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grin1 Mutation: 64 strains or lines available

References

• Original: J:38582 Tokita Y, et al., Characterization of excitatory amino acid neurotoxicity in N-methyl-D-aspartate receptor-deficient mouse cortical neuronal cells. Eur J Neurosci. 1996 Jan;8(1):69-78
• All: 1 reference(s)