Prox1^{tm3(cre/ERT2)Gco}
Targeted Allele Detail

Summary

• Symbol: Prox1^{tm3(cre/ERT2)Gco}
• Name: prospero homeobox 1; targeted mutation 3, Guillermo Oliver
• MGI ID: MGI:3759501
• Synonyms: Prox1^CreER, Prox-1CreER^T2, Prox1-CreER^T2, Prox1^ert2cre, Prox1^{tm3(cre/ERT2)Gco}, Prox1^{tm3(cre/ESR1)Gco}
• Gene: Prox1 Location: Chr1:189850232-189902911 bp, - strand Genetic Position: Chr1, 95.85 cM
• Alliance: Prox1^{tm3(cre/ERT2)Gco} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125507
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Prox1^{tm3(cre/ERT2)Gco} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prox1 (MGI:97772)

• Mutation details: A synthetic splice acceptor site, exons 3 and 4, and an IRES-cre/ERT2 cassette were inserted into intron 2 along with a neo and thymidine kinase cassettes. Insertion was confirmed by Southern blot analysis and through crosses with reporter lines. Expression of cre is tamoxifen inducible and recapitulates endogenous promoter activity specifically in the central nervous system, eye, liver, heart, branchial arches and lymphatic endothelial cells. (J:125507)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prox1 (mouse) Summary of all recombinase alleles driven by Prox1. Comparative matrix view of recombinase activities Alliance mouse Prox1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prox1 Mutation: 43 strains or lines available

References

• Original: J:125507 Srinivasan RS, et al., Lineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature. Genes Dev. 2007 Oct 1;21(19):2422-2432
• All: 67 reference(s)