Sim1^tm1.2Az
Targeted Allele Detail

Summary

• Symbol: Sim1^tm1.2Az
• Name: single-minded family bHLH transcription factor 1; targeted mutation 1.2, Andrew R Zinn
• MGI ID: MGI:3758479
• Synonyms: 2-loxP, Sim1-floxed exon 1 (PGK-Neo excised)
• Gene: Sim1 Location: Chr10:50770850-50865248 bp, + strand Genetic Position: Chr10, 24.87 cM, cytoband B3-B4
• Alliance: Sim1^tm1.2Az page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95410
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to place a loxP site just upstream of the ATG translational start site and a loxP-flanked PGK-Neo cassette downstream of the same exon of the targeted gene. The construct was injected into RW-4 ES cells. Chimeric mice were bred to C57BL/6 mice, and the neomycin selection cassette was excised from these mice by crossing to FVB transgenic mice with widespread Cre recombinase expression. (J:95410)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sim1 Mutation: 52 strains or lines available

References

• Original: J:95410 Holder JL Jr, et al., Sim1 gene dosage modulates the homeostatic feeding response to increased dietary fat in mice. Am J Physiol Endocrinol Metab. 2004 Jul;287(1):E105-13
• All: 3 reference(s)