Rr158^tm2Xu
Targeted Allele Detail

Summary

• Symbol: Rr158^tm2Xu
• Name: regulatory region 158; targeted mutation 2, Yang Xu
• MGI ID: MGI:3758449
• Synonyms: EkappaNI, Igk-Eik^tm2Xu, MiEkappa^-
• Gene: Rr158 Location: Chr6:70702935-70702935 bp Genetic Position: Chr6, Syntenic
• Alliance: Rr158^tm2Xu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124407
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The entire 740bp light chain intronic enhancer, including the MAR (matrix attachment site) (Ekappa, located between Igkj and Igkc gene segment clusters) was replaced with a loxP site flanked neomycin resistance gene cassette. A Southern blot was used to confirm the replacement. (J:32727)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr158 Mutation: 0 strains or lines available

References

• Original: J:124407 Inlay MA, et al., Critical roles of the immunoglobulin intronic enhancers in maintaining the sequential rearrangement of IgH and Igk loci. J Exp Med. 2006 Jul 10;203(7):1721-32
• All: 5 reference(s)