Esrrb^tm1.1Nat
Targeted Allele Detail

Summary

• Symbol: Esrrb^tm1.1Nat
• Name: estrogen related receptor, beta; targeted mutation 1.1, Jeremy Nathans
• MGI ID: MGI:3720481
• Synonyms: CKO^flp, Nr3b2^CKO
• Gene: Esrrb Location: Chr12:86407891-86568402 bp, + strand Genetic Position: Chr12, 40.49 cM
• Alliance: Esrrb^tm1.1Nat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124941
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to place a loxp site 184 bp upstream, as well as a loxP site 123 bp downstream of exon 2 of the targeted gene. An FRT-flanked PGK-neo cassette was also placed just downstream of the 3' loxP site. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6 (to generate Nr3b2^CKO+neo mice). The neomycin selection cassette was excised by crossing to mice constitutively expressing the Flp recombinase (unspecified genetic background, see Stock No. 003800), generating this Nr3b2^CKO allele with a single FRT site remaining. (J:124941)

Disease models

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esrrb Mutation: 211 strains or lines available

References

• Original: J:124941 Chen J, et al., Estrogen-Related Receptor beta/NR3B2 Controls Epithelial Cell Fate and Endolymph Production by the Stria Vascularis. Dev Cell. 2007 Sep;13(3):325-37
• All: 6 reference(s)