Cacna1a^{tm2(CACNA1A)Ttan}
Targeted Allele Detail

Summary

• Symbol: Cacna1a^{tm2(CACNA1A)Ttan}
• Name: calcium channel, voltage-dependent, P/Q type, alpha 1A subunit; targeted mutation 2, Tsutomu Tanabe
• MGI ID: MGI:3718034
• Synonyms: HS13ki(+)
• Gene: Cacna1a Location: Chr8:85065268-85366875 bp, + strand Genetic Position: Chr8, 40.95 cM
• Alliance: Cacna1a^{tm2(CACNA1A)Ttan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:123243
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette flanked by loxP and lox71 was inserted into exon 1. A knock-in vector containing plox66-HS13NP was inserted along with a cre-expressing plasmid, resulting in the replacement of the neo with the vector. The vector contained lox66, IRES, human cDNA encoding the calcium channel, voltage-dependent, P/Q type, alpha 1A subunit gene from HS13NP (GenBank AB035727), a polyA signal and an FRT-flanked CAG-hygro gene. The human gene contained 13 polyglutamine repeats. (J:123243)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna1a Mutation: 118 strains or lines available

References

• Original: J:123243 Saegusa H, et al., Properties of human Cav2.1 channel with a spinocerebellar ataxia type 6 mutation expressed in Purkinje cells. Mol Cell Neurosci. 2007 Feb;34(2):261-70
• All: 1 reference(s)