Tg(Gfap-APOE_i4*L28P)12Hube
Transgene Detail

Summary

• Symbol: Tg(Gfap-APOE_i4*L28P)12Hube
• Name: transgene insertion 12, Gerda Huber
• MGI ID: MGI:3717392
• Synonyms: AD61, ApoE4(L28P;C112R), ApoE4(L28P:C112R), Tg(Gfap-APOE*4*L28P)12Hube
• Transgene: Tg(Gfap-APOE_i4*L28P)12Hube Location: unknown
• Alliance: Tg(Gfap-APOE_i4*L28P)12Hube page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gfap-APOE_i4*L28P)12Hube expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

  Transgene codes for a protein with a leucine to proline mutation.

• Mutation details: The cDNA encoding human APOE isoform 4 carrying the substitution C112R was mutated at residue 28 from leucine to proline (L28P) by site-specific mutagenesis. The cDNA was cloned into a vector downstream of the rat glial fibrillary acid protein (Gfap) promoter and regulatory elements and upstream of the human beta-globin splice and polyadenylation sites. The linearized construct was injected into the male pronuclei of B6D2F1 zygotes. (J:100970)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:100970 Huber G, et al., Characterization of transgenic mice expressing apolipoprotein E4(C112R) and apolipoprotein E4(L28P; C112R). Neuroscience. 2000;101(1):211-8
• All: 1 reference(s)