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Targeted Allele Detail
Symbol: Gfra1tm2.1Jmi
Name: glial cell line derived neurotrophic factor family receptor alpha 1; targeted mutation 2.1, Jeffrey Milbrandt
MGI ID: MGI:3715157
Synonyms: Gfra1GFP
Gene: Gfra1  Location: Chr19:58224036-58444341 bp, - strand  Genetic Position: Chr19, 54.18 cM, cytoband D2-D3
Alliance: Gfra1tm2.1Jmi page
Germline Transmission:  Earliest citation of germline transmission: J:122607
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
Mutation detailsA loxP site flanked Gfra1 cDNA (with SV40 poly(A) signal sequences), a GFP gene (with poly(A) signal sequences), and an FRT site flanked neomycin resistance gene cassette were introduced into exon 2. 95 Nucleotides containing the 5' UTR, the initiator ATG codon and the signal sequences, were deleted. The neo cassette was removed through subsequent flp-mediated recombination. The inserted cDNA was deleted through cre-mediated recombination, thus abolishing any Gfra1 expression from this allele, but allowing expression of the GFP gene. (J:122607)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 26 assay results
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gfra1 Mutation:  25 strains or lines available
Original:  J:122607 Uesaka T, et al., Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. Development. 2007 Jun;134(11):2171-81
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory