Gfra1^tm2.1Jmi
Targeted Allele Detail

Summary

• Symbol: Gfra1^tm2.1Jmi
• Name: glial cell line derived neurotrophic factor family receptor alpha 1; targeted mutation 2.1, Jeffrey Milbrandt
• MGI ID: MGI:3715157
• Synonyms: Gfra1^GFP
• Gene: Gfra1 Location: Chr19:58224036-58444341 bp, - strand Genetic Position: Chr19, 54.18 cM, cytoband D2-D3
• Alliance: Gfra1^tm2.1Jmi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122607
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site flanked Gfra1 cDNA (with SV40 poly(A) signal sequences), a GFP gene (with poly(A) signal sequences), and an FRT site flanked neomycin resistance gene cassette were introduced into exon 2. 95 Nucleotides containing the 5' UTR, the initiator ATG codon and the signal sequences, were deleted. The neo cassette was removed through subsequent flp-mediated recombination. The inserted cDNA was deleted through cre-mediated recombination, thus abolishing any Gfra1 expression from this allele, but allowing expression of the GFP gene. (J:122607)

Expression

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	2 anatomical structures

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gfra1 Mutation: 25 strains or lines available

References

• Original: J:122607 Uesaka T, et al., Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. Development. 2007 Jun;134(11):2171-81
• All: 13 reference(s)