Ppp5c^Gt(XG029)Byg
Gene trapped Allele Detail

Summary

• Symbol: Ppp5c^Gt(XG029)Byg
• Name: protein phosphatase 5, catalytic subunit; gene trap mutation XG029, BayGenomics
• MGI ID: MGI:3715027
• Synonyms: Ppp5^-
• Gene: Ppp5c Location: Chr7:16738575-16761812 bp, - strand Genetic Position: Chr7, 9.15 cM
• Alliance: Ppp5c^Gt(XG029)Byg page

Mutation origin

• Mutant Cell Line: XG029
• Germline Transmission: Earliest citation of germline transmission: J:122564
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A beta-galactosidase gene flanked by a splice acceptor and a splice donor (from the pGT1Lxf vector) was inserted into intron 1 creating a null allele. The absence of protein product was confirmed by western blot analysis. (J:122564)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XG029	CC182963 ; (MGI Seq Detail)	5' RACE	Chr7:16761619-16761753 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  16 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp5c Mutation: 46 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:122564 Yong W, et al., Mice lacking protein phosphatase 5 are defective in ataxia telangiectasia mutated (ATM)-mediated cell cycle arrest. J Biol Chem. 2007 May 18;282(20):14690-4
• All: 5 reference(s)