Tg(CAG-Ngb,-EGFP)1Dgrn
Transgene Detail

Summary

• Symbol: Tg(CAG-Ngb,-EGFP)1Dgrn
• Name: transgene insertion 1, David Greenberg
• MGI ID: MGI:3714625
• Synonyms: Ngb-Tg, Tg(ACTB-Ngb,-EGFP)1Dgrn, Tg(CAG-Ngb,-EGFP)1Dgrn
• Transgene: Tg(CAG-Ngb,-EGFP)1Dgrn Location: unknown
• Alliance: Tg(CAG-Ngb,-EGFP)1Dgrn page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1 x CD-1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-Ngb,-EGFP)1Dgrn expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ngb (MGI:2151886)

• Mutation details: A transgenic construct containing the mouse neuroglobin gene, Ngb and an Enhanced Green Fluorescent Protein gene under the control of the chicken beta actin (ACTB) promoter coupled with the cytomegalovirus (CMV) distal enhancer was introduced into outbred (C57BL/6 x DBA/2)F1 x CD-1 donor eggs. Founder line 1 was subsequently established. Constitutive Ngb protein expression is increased in the brain and heart (in vascular endothelial cells) of transgenic mice, as shown by Western blot. Immunohistochemistry shows increased numbers of neurons, astrocytes and endothelial cells expressing Ngb protein. (J:117158)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:117158 Khan AA, et al., Neuroglobin-overexpressing transgenic mice are resistant to cerebral and myocardial ischemia. Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17944-8
• All: 6 reference(s)