Aqp2^tm1(cre)Blyg
Targeted Allele Detail

Summary

• Symbol: Aqp2^tm1(cre)Blyg
• Name: aquaporin 2; targeted mutation 1, Baoli Yang
• MGI ID: MGI:3711456
• Synonyms: Aqp^delta230
• Gene: Aqp2 Location: Chr15:99476937-99482426 bp, + strand Genetic Position: Chr15, 56.13 cM
• Alliance: Aqp2^tm1(cre)Blyg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121445
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Aqp2^tm1(cre)Blyg expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aqp2 (MGI:1096865)

• Mutation details: A targeting vector was designed to insert an IRES-cre-ER-neo into exon 4. This insertion resulted in the loss of the protein kinase A phosphorylation site (S256), but the putative apical localization signal remained. RT-PCR demonstrates no expression for the downstream segment of exon 4, however, transcript for the upstream portion including cre recombinase, was detected. (J:121445)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aqp2 (mouse) Summary of all recombinase alleles driven by Aqp2. Alliance mouse Aqp2 gene page

Disease models

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aqp2 Mutation: 17 strains or lines available

References

• Original: J:121445 Shi PP, et al., Nephrogenic diabetes insipidus in mice caused by deleting COOH-terminal tail of aquaporin-2. Am J Physiol Renal Physiol. 2007 May;292(5):F1334-44
• All: 1 reference(s)