Tg(HHV8ORF73,TK-GFP)1Dtmr
Transgene Detail

Summary

• Symbol: Tg(HHV8ORF73,TK-GFP)1Dtmr
• Name: transgene insertion 1, Dirk P Dittmer
• MGI ID: MGI:3709363
• Synonyms: LANA transgene
• Transgene: Tg(HHV8ORF73,TK-GFP)1Dtmr Location: unknown
• Alliance: Tg(HHV8ORF73,TK-GFP)1Dtmr page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The bicistronic transgene contains the protein-coding sequence of ORF73 from human herpesvirus 8 (HHV-8; commonly known as Kaposi sarcoma-associated herpesvirus, KSHV), encoding the viral latency-associated nuclear antigen (LANA), downstream of 1299 bp of viral DNA containing the native ORF73 5' regulatory sequences; the cellular thymidine kinase (Tk) promoter followed by the green fluorescent protein (GFP) coding sequence and an SV40 polyadenylation signal reside downstream of the LANA coding sequence. Northern blot analysis detects ORF73 mRNA in spleens and sometimes kidneys of transgenic mice, and immunohistochemistry demonstrates presence of LANA in proliferating cell nuclear antigen-(PCNA-)positive cells in the central region of splenic follicles. No GFP fluorescence has been detected in the transgenic mice. (J:106515)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Per J:106515, "Multiple independent founder lines were generated by pronuclear injection." Lines i ii iii iv and v are mentioned; two lines were characterized phenotypically, but not identified/ named.

References

• Original: J:106515 Fakhari FD, et al., The latency-associated nuclear antigen of Kaposi sarcoma-associated herpesvirus induces B cell hyperplasia and lymphoma. J Clin Invest. 2006 Mar;116(3):735-42
• All: 2 reference(s)