Tg(CD19-BTK*Y223F)AM02Rwhe
Transgene Detail

Summary

• Symbol: Tg(CD19-BTK*Y223F)AM02Rwhe
• Name: transgene insertion AM02, Rudolf Hendriks
• MGI ID: MGI:3709258
• Synonyms: CD19-hBtk^Y223F
• Transgene: Tg(CD19-BTK*Y223F)AM02Rwhe Location: unknown
• Alliance: Tg(CD19-BTK*Y223F)AM02Rwhe page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD19-BTK*Y223F)AM02Rwhe expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BTK (695)

  Btk	(MGI:88216)

  Transgene codes for a protein with a tyrosene to phenylalanine mutation.

• Mutation details: A composite fragment composed of 400bp from exons 1-3 of a BTK cDNA as well as 27kb genomic fragment containing exons 3-19 of BTK was placed under the control of the promoter region of human CD19. A Y223F mutation was introduced into this construct by double stranded site-directed mutagenesis. (J:120989)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:120989 Middendorp S, et al., Function of Bruton's tyrosine kinase during B cell development is partially independent of its catalytic activity. J Immunol. 2003 Dec 1;171(11):5988-96
• All: 2 reference(s)