Nckap1^Gt(XE068)Byg
Gene trapped Allele Detail

Summary

• Symbol: Nckap1^Gt(XE068)Byg
• Name: NCK-associated protein 1; gene trap XE068, BayGenomics
• MGI ID: MGI:3707168
• Synonyms: Nap1^GT
• Gene: Nckap1 Location: Chr2:80330856-80411526 bp, - strand Genetic Position: Chr2, 48.21 cM
• Alliance: Nckap1^Gt(XE068)Byg page

Mutation origin

• Mutant Cell Line: XE068
• Germline Transmission: Earliest citation of germline transmission: J:119028
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap vector containing a beta-geo was inserted 504 base pairs downstream of exon 24, creating a fusion of the 898 N-terminal amino acids with beta-geo. This ES cell line contains an identical insertion to line XE133. (J:119028)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE068	CC177958 ; (MGI Seq Detail)	5' RACE	Chr2:80348238-80350778 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  15 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nckap1 Mutation: 71 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:119028 Rakeman AS, et al., Axis specification and morphogenesis in the mouse embryo require Nap1, a regulator of WAVE-mediated actin branching. Development. 2006 Aug;133(16):3075-83
• All: 3 reference(s)