Flnb^{Gt(BGB085)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Flnb^{Gt(BGB085)Byg}
• Name: filamin, beta; gene trap BGB085, BayGenomics
• MGI ID: MGI:3704947
• Gene: Flnb Location: Chr14:14518185-14651816 bp, - strand Genetic Position: Chr14, 4.59 cM
• Alliance: Flnb^{Gt(BGB085)Byg} page

Skeletal abnormalities in Flnb^{Gt(BGB085)Byg}/Flnb^{Gt(BGB085)Byg} mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: BGB085
• Germline Transmission: Earliest citation of germline transmission: J:120066
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A BayGenomics gene trap vector (pGT1lxf) was inserted into intron 20 to create an in frame 5' fusion product with a lacZ cassette. The absence of the 250KDa protein was confirmed by western blot with an antibody recognizing the C-terminus end of Flnb. A beta-galactosidase assay confirmed expression of the lacZ fusion product. (J:120066)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  BGB085	CL903412 ; (MGI Seq Detail)	5' RACE	Chr14:14545460-14545510 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  93 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Flnb Mutation: 155 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:120066 Zhou X, et al., Filamin B deficiency in mice results in skeletal malformations and impaired microvascular development. Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3919-24
• All: 2 reference(s)