Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe
Transgene Detail

Summary

• Symbol: Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe
• Name: transgene insertion 1, Corinne G Lobe
• MGI ID: MGI:3703057
• Synonyms: IC-Notch1, Notch1-GFP, Tg(ACTB-Bgeo,-NOTCH1,-EGFP)1Lbe, Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe, Z/EG-Notch
• Transgene: Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe Location: unknown
• Alliance: Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:83045
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  NOTCH1 (4851)

  Notch1

  (MGI:97363)

• Mutation details: A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), Myc-tagged intracellular portion of human NOTCH1 (IC-Notch), internal ribosomal entry site, and an enhanced green fluorescent protein (EGFP) gene. Prior to cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells and enabling tissue-specific studies of NOTCH expression using appropriate Cre mice. (J:83045, J:119129)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:119129 Liu J, et al., Constitutive notch signaling in adult transgenic mice inhibits bFGF-induced angiogenesis and blocks ovarian follicle development. MGI Direct Data Submission. 2007
• All: 14 reference(s)