Kmt2a^{tm1.1(Sh3gl1)Lcc}
Targeted Allele Detail

Summary

• Symbol: Kmt2a^{tm1.1(Sh3gl1)Lcc}
• Name: lysine (K)-specific methyltransferase 2A; targeted mutation 1.1, Li Chong Chan
• MGI ID: MGI:3701943
• Synonyms: Mll^EenINV
• Gene: Kmt2a Location: Chr9:44714652-44792594 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Kmt2a^{tm1.1(Sh3gl1)Lcc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205605
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, No functional change)
• Mutations: Insertion, Intragenic deletion
• Kmt2a^{tm1.1(Sh3gl1)Lcc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sh3gl1 (MGI:700010)	fusion protein created after cre-mediated recombination

• Mutation details: A cassette containing a splice acceptor, the open reading frame of Sh3gl1, an IRES-EGFP and an FRT-flanked neomycin resistance cassette was flanked by inward facing loxP sites and inserted in opposite orientation between exon 8 and 9. Flp-mediated recombination removed the selection cassette. Cre-mediated inversion is required to achieve expression of the fusion at exon 8. The fusion products mimick a fusion found in human patients with leukemia. (J:205605)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2a Mutation: 137 strains or lines available

Notes

ES cell line = L4.

References

• Original: J:205605 Ng RK, et al., Epigenetic dysregulation of leukaemic HOX code in MLL-rearranged leukaemia mouse model. J Pathol. 2014 Jan;232(1):65-74
• All: 3 reference(s)