Ncf4^tm1Pth
Targeted Allele Detail

Summary

• Symbol: Ncf4^tm1Pth
• Name: neutrophil cytosolic factor 4; targeted mutation 1, Phillip T Hawkins
• MGI ID: MGI:3701805
• Synonyms: p40^phoxR58A
• Gene: Ncf4 Location: Chr15:78129001-78146780 bp, + strand Genetic Position: Chr15, 37.1 cM
• Alliance: Ncf4^tm1Pth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119217
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The sequence of codon 58 was altered from CGC to GCC resulting in an arginine to alanine amino acid substitution (p.R58A) that disrupts PtdIns3P binding. A self-excising floxed tACE-Cre expression module and neo cassette was inserted into intron 3, and was removed by cre-mediated excision specifically in the testis during breeding of the chimeras. (J:119217)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncf4 Mutation: 25 strains or lines available

References

• Original: J:119217 Ellson C, et al., PtdIns3P binding to the PX domain of p40phox is a physiological signal in NADPH oxidase activation. EMBO J. 2006 Oct 4;25(19):4468-78
• All: 6 reference(s)