Gck^tm2Mgn
Targeted Allele Detail

Summary

• Symbol: Gck^tm2Mgn
• Name: glucokinase; targeted mutation 2, Mark A Magnuson
• MGI ID: MGI:3701697
• Synonyms: GK(K414E)
• Gene: Gck Location: Chr11:5850820-5900081 bp, - strand Genetic Position: Chr11, 3.88 cM
• Alliance: Gck^tm2Mgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121910
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: A construct with an inactivating single base pair mutation was introduced into exon 9 via site specific mutagenesis. This is predicted to to change amino acid 414 from lysine to glutamic acid in the encoded protein. (J:121910)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gck Mutation: 69 strains or lines available

Notes

On congenic B6 background, analysis is done on mice with allele with pgk-neo selection cassette deleted through mating to cre transgenic mice; on the coisogenic 129S6 background, allele retaining pgk-neo is analyzed, and authors state that presence of neo has no apparent effect on phenotype compared with mice on C57BL/6J congenic background. MMRRC has 129S6 mice carrying Gck^tm2Mgn (retaining neo cassette).

References

• Original: J:121910 Pino MF, et al., Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations. J Biol Chem. 2007 May 4;282(18):13906-16
• All: 1 reference(s)