Mecp2tm2Bird
Targeted Allele Detail
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Symbol: |
Mecp2tm2Bird |
Name: |
methyl CpG binding protein 2; targeted mutation 2, Adrian Bird |
MGI ID: |
MGI:3700191 |
Synonyms: |
Mecp2lox-stop, Mecp2stop |
Gene: |
Mecp2 Location: ChrX:73070198-73129296 bp, - strand Genetic Position: ChrX, 37.63 cM
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Alliance: |
Mecp2tm2Bird page
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Germline Transmission: |
Earliest citation of germline transmission:
J:118365
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Parent Cell Line: |
E14TG2a (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Conditional ready, Null/knockout) |
Mutation: |
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Insertion
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Mutation details: Cre recombinase-reversible gene inactivation was accomplished by insertion into intron 2 of a 3.1-kb DNA fragment containing a loxP-flanked "neostop" cassette, which comprises a neomycin resistance cassette followed by a transcriptional/translational "stop" cassette composed of 550 bp of 3' sequence from the Saccharomyces cerevisiae His3 gene, an SV40 polyadenylation sequence, and a synthetic sequence containing a false translation initiation codon (ATG) immediately followed by a consensus splice donor sequence. Despite the production of a read-through transcript, no protein product was detected by western blot or immunofluorescence analysis of brains of mutant mice.
(J:75205, J:111323, J:118365)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:118365 Guy J, et al., Reversal of neurological defects in a mouse model of Rett syndrome. Science. 2007 Feb 23;315(5815):1143-7 |
All: |
40 reference(s) |
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