Grin2b^{tm1(GRIN2A/GRIN2B)Twr}
Targeted Allele Detail

Summary

• Symbol: Grin2b^{tm1(GRIN2A/GRIN2B)Twr}
• Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2); targeted mutation 1, Thomas W Rosahl
• MGI ID: MGI:3699608
• Gene: Grin2b Location: Chr6:135690231-136150509 bp, - strand Genetic Position: Chr6, 66.38 cM
• Alliance: Grin2b^{tm1(GRIN2A/GRIN2B)Twr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:116524
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert the 6 kb long human chimeric cDNA clone GRIN2A/GRIN2B in-frame, leaving the 34 amino-acid long signal peptide sequence intact. Amino acid residues 35-282 of the human glutamate receptor, ionotropic, NMDA2A (epsilon 1) and the carboxyl terminal remainder of the human glutamate receptor, ionotropic, NMDA2B (epsilon 2) residues were inserted into the mouse locus. The vector was inserted into exon 4 (the first coding exon) of the endogenous locus. (J:116524)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grin2b Mutation: 96 strains or lines available

References

• Original: J:116524 Rosahl TW, et al., A genetically modified mouse model probing the selective action of ifenprodil at the N-methyl-D-aspartate type 2B receptor. Mol Cell Neurosci. 2006 Sep;33(1):47-56
• All: 1 reference(s)