Syt7^tm2Sud
Targeted Allele Detail

Summary

• Symbol: Syt7^tm2Sud
• Name: synaptotagmin VII; targeted mutation 2, Thomas C Sudhof
• MGI ID: MGI:3699592
• Synonyms: C2B KI/KO
• Gene: Syt7 Location: Chr19:10366454-10430544 bp, + strand Genetic Position: Chr19, 6.58 cM, cytoband B
• Alliance: Syt7^tm2Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118319
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A targeting vector containing point mutations D303A, D357A, and D359A in exon 13 as well as a floxed neomycin gene in intron 12 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. The neomycin gene was removed by crossing with a Cre deleter strain, which left the expression level at 20-30% of wild-type. (J:118319, J:131423)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Syt7 Mutation: 38 strains or lines available

References

• Original: J:118319 Sudhof TC, A modified knock-in mutation of Syt7. MGI Direct Data Submission. 2007
• All: 7 reference(s)