Tg(Ext2*D227N,lacZ)M13Tsh
Transgene Detail

Summary

• Symbol: Tg(Ext2*D227N,lacZ)M13Tsh
• Name: transgene insertion M13, Takuji Shirasawa
• MGI ID: MGI:3698184
• Synonyms: Tg(Ext2(ASP227ASN),lacZ)M13Tsh, TgEXT2(D227N)/lacZ
• Transgene: Tg(Ext2*D227N,lacZ)M13Tsh Location: unknown
• Alliance: Tg(Ext2*D227N,lacZ)M13Tsh page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ext2*D227N,lacZ)M13Tsh expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ext2 (MGI:108050)

• Mutation details: Two co-injected transgene constructs both employ the regulatory sequences of the mouse procollagen, type XI, alpha 2 (Col11a2) gene, in a DNA fragment encompassing nucleotides -742 through +380 (relative to the transcription start site), to direct expression to developing chondrocytes. One construct contains a full-length, 2.2-kb cDNA encoding a mutant mouse exostoses (multiple) 2 protein with the hereditary multiple exostoses- (HME-) associated aspartic acid-to-asparagine substitution at amino acid position 227, followed by the SV40 splice donor/acceptor and polyadenylation signal. In the second construct, the mouse cDNA is replaced by the E. coli beta-galactosidase (lacZ) coding sequence. (J:48125, J:75965)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Embryonic and adult mice of 3 high-copy number transgenic lines were analyzed; no distinction was made among them.

References

• Original: J:75965 Morimoto Ki K, et al., Transgenic Expression of the EXT2 Gene in Developing Chondrocytes Enhances the Synthesis of Heparan Sulfate and Bone Formation in Mice. Biochem Biophys Res Commun. 2002 Apr 12;292(4):999-1009
• All: 2 reference(s)