Gata3^tm3Gsv
Targeted Allele Detail

Summary

• Symbol: Gata3^tm3Gsv
• Name: GATA binding protein 3; targeted mutation 3, Frank G Grosveld
• MGI ID: MGI:3696958
• Synonyms: Gata3^cko, Gata3^fl
• Gene: Gata3 Location: Chr2:9861889-9894845 bp, - strand Genetic Position: Chr2, 6.69 cM
• Alliance: Gata3^tm3Gsv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:117058
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert loxP sites into the start codon in exon 2 and in intron 2. A puromycin resistance cassette, flanked by FRT sites was coupled to an nlsLacZ gene in intron 2. Transient expression of Flp recombinase resulted in removal of the puro cassette. Upon cre mediated excision, the 5' end of exon 2 is removed as well as intron 2 sequence upstream of the nlslacZ. This allows the reporter to be expressed under endogenous control. (J:117058)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	17 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata3 Mutation: 31 strains or lines available

References

• Original: J:117058 Kurek D, et al., Transcriptome and phenotypic analysis reveals Gata3-dependent signalling pathways in murine hair follicles. Development. 2007 Jan;134(2):261-72
• All: 12 reference(s)