Gt(ROSA)26Sor^{tm4(Ikbkb)Rsky}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(Ikbkb)Rsky}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Klaus Rajewsky
• MGI ID: MGI:3687199
• Synonyms: Gt(ROSA)26Sor^{tm1(Ikbkb)Mass}, R26IKK2ca^sFL, R26Stop^FLikk2ca, R26StopIkk2SSEE
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(Ikbkb)Rsky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:113365
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(Ikbkb)Rsky} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Mouse	Ikbkb

• Mutation details: An IRES-eGFP cassette and a cDNA sequence encoding Ikbkb containing two serine to glutamate substitutions in the activation loop of the kinase domain, preceded by a floxed stop-neo cassette was inserted into the locus. The Ikbkb would be expressed under the control of the endogenous ROSA26 promoter after cre-mediated excision of the STOP cassette. (J:113365)

Disease models

Expression

In Mice Carrying this Mutation:	37 assay results
In Structures Affected by this Mutation:	2 anatomical structures

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm4(Ikbkb)Rsky}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 795 strains or lines available

References

• Original: J:113365 Sasaki Y, et al., Canonical NF-kappaB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Immunity. 2006 Jun;24(6):729-39
• All: 38 reference(s)