Igh^tm11.1Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm11.1Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 11.1, University of Cologne
• MGI ID: MGI:3687002
• Synonyms: D23^stopVDJ
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:113434
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Igh^tm11.1Cgn involves 1 genes/genome features (Igh) View all
• Mutation details: A targeting vetor was used to replace the JH1 and JH2 elements with the D23^stop joint. A floxed neo included in the vector was subsequently removed by transient transfection of cre recombinase. A second targeting vector was then used to introduce a double loxP site and frt-flanked neo into the VH81x position. Crossing these mice with cre deleter mice results in the loss of the DH elements and the insertion of a D23^stop VDJ cassette. (J:113434)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 45 strains or lines available

References

• Original: J:113434 Koralov SB, et al., Antibody repertoires generated by VH replacement and direct VH to JH joining. Immunity. 2006 Jul;25(1):43-53
• All: 1 reference(s)