Ssbp3^{Gt(RRB078)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ssbp3^{Gt(RRB078)Byg}
• Name: single-stranded DNA binding protein 3; gene trap RRB078, BayGenomics
• MGI ID: MGI:3663396
• Synonyms: Ssbp3^{Gt(pGT1Lxf)8Byg}, Ssdp1²⁸⁵
• Gene: Ssbp3 Location: Chr4:106768667-106906891 bp, + strand Genetic Position: Chr4, 49.9 cM, cytoband C7
• Alliance: Ssbp3^{Gt(RRB078)Byg} page

Mutation origin

• Mutant Cell Line: RRB078
• Germline Transmission: Earliest citation of germline transmission: J:111801
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (No functional change)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A Beta-geo gene trap vector, pGT1Lxf, was inserted into intron 13 of the locus. The first 285 amino acids were fused to the Beta-galactosidase-neomycin protein. This allele retains most of the proline-rich stretch as it loses only the C-terminal 103 residues. Western blots demonstrate expression of chimeric protein composed of the endogenous N-terminal polypeptide fused to Beta-geo. (J:111801)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRB078	CC183248 ; (MGI Seq Detail)	5' RACE	Chr4:106885386-106895973 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  46 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ssbp3 Mutation: 41 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:111801 Enkhmandakh B, et al., The role of the proline-rich domain of Ssdp1 in the modular architecture of the vertebrate head organizer. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11631-6
• All: 2 reference(s)