Gt(ROSA)26Sor^{tm1(Stat3)Cgn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Stat3)Cgn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, University of Cologne
• MGI ID: MGI:3655484
• Synonyms: Gt(ROSA)26Sor^{tm1(Stat3,EGFP)Cgn}, R26^LSL-Stat3C, R26-Stat3c^fl-STOP, Stat3-C^FL
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Stat3)Cgn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109583
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: mixed

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Stat3)Cgn} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Stat3 (MGI:103038)

• Mutation details: ES cells from Ighg1^{tm1(IRES-cre)Cgn} heterozygous mice were targeted at the ROSA26 locus with a conditional transgene encoding a constitutively active form of the STAT-3 protein proceded by a floxed STOP cassette and followed by an IRES-EGFP cassette. Upon SRBC immunization, STAT-3c expression is detected in the majority of germinal centers and IgG1 memory B cells by EGFP expression. (J:109583)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

Notes

ES cell lines were established from Ighg1^{tm1(IRES-cre)Cgn} heterozygous mice on the (C57BL/6 x BALB/c)F1 genetic background. Such ES cells can be used for further genetic manipulation and then injected into tetraploid blastocysts to obtain cloned mice for analysis.

References

• Original: J:109583 Casola S, et al., Tracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting. Proc Natl Acad Sci U S A. 2006 May 9;103(19):7396-401
• All: 2 reference(s)