Tirap^tm1Aki
Targeted Allele Detail

Summary

• Symbol: Tirap^tm1Aki
• Name: toll-interleukin 1 receptor (TIR) domain-containing adaptor protein; targeted mutation 1, Shizuo Akira
• MGI ID: MGI:3654146
• Synonyms: Mal^-, Tirap-
• Gene: Tirap Location: Chr9:35095847-35111587 bp, - strand Genetic Position: Chr9, 19.99 cM, cytoband A5.3
• Alliance: Tirap^tm1Aki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111205
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details:

  A targeting vector was designed to replace the two exons encoding the TIR domain with a neomycin resistance gene. The insertion created a stop codon in the N-terminal portion of the locus. Immunoblot confirmed that disruption of the locus abolished the expression of protein. (J:111205)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tirap Mutation: 16 strains or lines available

References

• Original: J:111205 Yamamoto M, et al., Essential role for TIRAP in activation of the signalling cascade shared by TLR2 and TLR4. Nature. 2002 Nov 21;420(6913):324-9
• All: 36 reference(s)