Map2^tm1Zhku
Targeted Allele Detail

Summary

• Symbol: Map2^tm1Zhku
• Name: microtubule-associated protein 2; targeted mutation 2, Z Khuchua
• MGI ID: MGI:3653757
• Synonyms: delta1-158
• Gene: Map2 Location: Chr1:66214432-66481742 bp, + strand Genetic Position: Chr1, 33.49 cM
• Alliance: Map2^tm1Zhku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110727
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exon 2 with a neomycin resistance gene. This exon was 285-bp long and contained the translation start site and the RII bonding site. Mutants would putatively generate a truncated protein, translated from the next in-frame ATG site in exon 3. Western blot demonstrated that mutants expressed more Mtap2 antigen than wild-type animals. Transcript levels were also increased, suggesting that some modulating regulatory elements are present within the targeted sequence deleted from the locus. (J:110727)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map2 Mutation: 109 strains or lines available

References

• Original: J:110727 Khuchua Z, et al., Deletion of the N-terminus of murine map2 by gene targeting disrupts hippocampal ca1 neuron architecture and alters contextual memory. Neuroscience. 2003;119(1):101-11
• All: 1 reference(s)