Tg(Gfap-EGFP)9Shb
Transgene Detail
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| Symbol: |
Tg(Gfap-EGFP)9Shb |
| Name: |
transgene insertion 9, Satoru Arata |
| MGI ID: |
MGI:3641542 |
| Synonyms: |
line #9, Tg(GFAP-EGFP)09Shb |
| Transgene: |
Tg(Gfap-EGFP)9Shb Location: unknown
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| Alliance: |
Tg(Gfap-EGFP)9Shb page
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: This transgene joins the genomic sequence comprising nucleotides -2567 through +12, relative to the transcription initiation site, of the mouse glial fibrillary acidic protein promoter to the cDNA encoding enhanced green fluorescent protein (EGFP) followed by a rabbit beta-globin polyadenylation signal. No EGFP fluorescence is detectable in fixed slices of most brain regions of unmanipulated mice; a few fluorescent cells occur in the hippocampi of only some animals. Following a needle-inflicted wound to the brain, fluorescent cells with morphologic characteristics and surface markers (vimentin and GFAP) of reactive glia, but not of microglia (CD11b) or neurons (NeuN), become apparent in the region surrounding the wound. In living brain slices, EGFP is expressed in cells with the appearance of protoplasmic, fibrous and reactive astrocytes. Fluorescence is visible
throughout these cells, including in astrocytic fine processes.
(J:110869, J:110870)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Two transgenic mice derived from oocyte injections transmitted the transgene via the germline. The resultant transgenic lines were designated #7 and #9. Line #9, which exhibited the greater fluorescence, was used in most experiments.
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| Original: |
J:110870 Suzuki R, et al., Expression of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in reactive astrocytes. Brain Res Mol Brain Res. 2003 Jul 4;115(1):10-20 |
| All: |
10 reference(s) |
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Analysis Tools
