Tg(Ins2-Cxcl13)1Cys
Transgene Detail

Summary

• Symbol: Tg(Ins2-Cxcl13)1Cys
• Name: transgene insertion 1, Jason G Cyster
• MGI ID: MGI:3641325
• Synonyms: RIP-BLC1, RIP-CXCL13
• Transgene: Tg(Ins2-Cxcl13)1Cys Location: unknown
• Alliance: Tg(Ins2-Cxcl13)1Cys page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-Cxcl13)1Cys expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Cxcl13 (MGI:1888499)

• Tg(Ins2-Cxcl13)1Cys expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgene comprises the full-length Cxcl13 (BLC) cDNA under control of the rat insulin 2 promoter. Immunostaining of adjacent pancreatic sections with antibodies to insulin and to CXCL13 demonstrated that all the beta cells express CXCL3; the intensity of staining for CXCL3 is several fold greater in pancreatic beta cells than in follicular stromal cells, where it is expressed from the endogenous gene. No expression is detected in liver or kidney. (J:77238, J:110548)
• Inheritance: Dominant

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three lines, having 4, 9 and 19 estimated copies of the transgene, were generated. Mice of all three lines exhibited similar B cell-enriched lymphocyte infiltration of the pancreatic beta cells. The line with 19 transgene copies, designated line 1, was used for further analyses.

References

• Original: J:110548 Luther SA, et al., BLC expression in pancreatic islets causes B cell recruitment and lymphotoxin-dependent lymphoid neogenesis. Immunity. 2000 May;12(5):471-81
• All: 3 reference(s)