Igh^{tm8(VHB1-8)Cgn}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm8(VHB1-8)Cgn}
• Name: immunoglobulin heavy chain complex; targeted mutation 8, University of Cologne
• MGI ID: MGI:3640326
• Synonyms: Igh^tm8Cgn, pdelta deltaneo
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48835
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Through homologous recombination a modified version of the vector used to create Igh^tm2Cgn replaced the endogenous sequence. This vector replaces a 3' Igh-D element (DQ52) and Igh-J elements with a recombined antibody variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8), includes a termination codon in the leader exon of B1-8 and deletes the endogenous promoter. (J:48835)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:48835 Fukita Y, et al., Somatic hypermutation in the heavy chain locus correlates with transcription. Immunity. 1998 Jul;9(1):105-14
• All: 3 reference(s)