Gt(ROSA)26Sor^{tm1(CAG-EGFP)Luo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-EGFP)Luo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Liqun Luo
• MGI ID: MGI:3623345
• Synonyms: GG, Gt(ROSA)26Sor^tm1(EGFP)Luo, Gt(ROSA)26Sor^tm1Luo, MADM GG knockin
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-EGFP)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98961
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A construct containing the CMV beta-actin enhancer/promoter, SV40 poly(A) signal, and encoding the N-terminal and C-terminal halves of a mutant enhanced green fluorescent protein (EGFP) separated by a beta-globin intron containing a loxP-flanked neomycin resistance gene, was knocked into ES cells at the ROSA26 locus. The two parts of the coding sequence are separated by the intron but still in the same reading frame. Ubiquitous GFP expression occurs in the absence of cre-mediated recombination. (J:98961)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:98961 Zong H, et al., Mosaic analysis with double markers in mice. Cell. 2005 May 6;121(3):479-92
• All: 4 reference(s)