Kmt2b^tm1Afst
Targeted Allele Detail

Summary

• Symbol: Kmt2b^tm1Afst
• Name: lysine (K)-specific methyltransferase 2B; targeted mutation 1, A Francis Stewart
• MGI ID: MGI:3623309
• Synonyms: Mll2^-
• Gene: Kmt2b Location: Chr7:30268283-30288151 bp, - strand Genetic Position: Chr7, 18.63 cM, cytoband A3
• Alliance: Kmt2b^tm1Afst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107403
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert an FRT flanked sequence containing the splice acceptor sequence from exon 2 of engrailed 2, EMCV IRES, a lacZ-neo cassette and SV40 polyA signal into intron 1. There was a loxP site on the 3' end and a second loxP site inserted into intron 2. RT-PCR confirmed that the cassette truncated the transcript before exon 2. A transcript encoding the first 121 amino acids of the locus fused to 40 frameshifted amino acids encoded by the second exon of engrailed 2 could be expressed. Transcript was not detected by RT-PCR of mutants. (J:107403)

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2b Mutation: 66 strains or lines available

Notes

Chimeras made using homozygous null ES cells where null cells are over 50% of the total cell population are absent by E18.5. Chimeras where null cells are less than 50% of the total cell population survive but the percentage of null cells decreases with age so that by E18.5 very few homozygous null cells are present, suggesting a cell autonomous defect.

References

• Original: J:107403 Glaser S, et al., Multiple epigenetic maintenance factors implicated by the loss of Mll2 in mouse development. Development. 2006 Apr;133(8):1423-32
• All: 5 reference(s)