Ptk2^tm1Ilic
Targeted Allele Detail

Summary

• Symbol: Ptk2^tm1Ilic
• Name: PTK2 protein tyrosine kinase 2; targeted mutation 1, Dusko Ilic
• MGI ID: MGI:3623143
• Synonyms: FAK^-
• Gene: Ptk2 Location: Chr15:73076951-73295129 bp, - strand Genetic Position: Chr15, 33.94 cM
• Alliance: Ptk2^tm1Ilic page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:24213
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A pgk1-neo cassette lacking a polyA signal was inserted into the exon spanning base pairs 1442 to 1525, which encode the beginning of the kinase domain. Northern blot confirmed absence of normal transcript as well as any truncated transcripts. Western blot failed to detect protein in mutant ES cells. (J:24213)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptk2 Mutation: 90 strains or lines available

References

• Original: J:24213 Ilic D, et al., Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells. Biochem Biophys Res Commun. 1995 Apr 6;209(1):300-9
• All: 4 reference(s)